Inter-laboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2’-deoxyguanosine MARCUS
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چکیده
Urinary 8-oxo-7,8-dihydro-2‟-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Herein we report the comparison of two, distinct chromatographic assays, with ELISA. Chromatographic assays displayed good agreement (r = 0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with ELISA (HPLC-GC/MS: r=0.43, HPLC-EC: r=0.56; p<0.0001). Mean values differed significantly between chromatographic assays and ELISA (HPLC-GC/MS: 3.86, HPLC-EC: 4.20, ELISA: 18.70 ng/mg creatinine, p<0.0001). Whilst it is reassuring to note good agreement between chromatographic assay, this study reveals significant short-comings in the ELISA, which bring into question its continued use in its present form.
منابع مشابه
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تاریخ انتشار 2009